Dibasic sodium phosphate dihydrate Cell culture 250GM Go Back


: 10028-24-7

Molecular Formula

: Na2HPO4.2H2O

Molecular Weight

: 177.99
Appearance: white crystals/crystalline powder.
Solubility: Soluble in water. practically insoluble in ethanol (96 per cent).
Identification (By chemical test): Solution should be slightly alkaline
Identification (Sodium): A dense precipitate should form
Identification (Phosphate 1): A yellow precipitate that solution in 2N nitric acid and in 6N ammonium hydroxide should form.
Identification (Phosphate 2): With ammonium molybdate solution, acidified solutions should yield a yellow precipitate which should soluble in 6N ammonium hydroxide
Identification (Phosphate 3): A yellow colour should develop
Appearance of solution: Clear and colourless solution
Water insoluble matter: NMT 0.3%
pH (5% aq soln): 8.9 – 9.2
Substances reducing permanganate: The colour of the permanganate is not completely discharged
Sodium dihydrogen phosphate: NMT 1.7%
Chloride (Cl): NMT 0.005%
Sulphate (SO4): NMT 0.02%
Arsenic (As): NMT 0.0002%
Iron (Fe): NMT 0.001%
Loss on drying at 130°C: 19.5% - 21.0%
Assay (on dry basis): 98.0% - 100.5%
Assay (Dried substance): 98.0% - 101.0% (Na2HPO4)
Total aerobic microbial count: NMT 100CFU/gm
Total combined molds and yeasts count: NMT 100CFU/gm
Bacterial endotoxin test: NMT 2.5 IU/gm
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