Disodium phosphate anhydrous Cell culture 180GM Go Back


: 7558-79-4

Molecular Formula

: Na2HPO4

Molecular Weight

: 141.96
Appearance/Description: White powder that readily absorbs moisture.
Solubility: Freely soluble in water; insoluble in alcohol
Identification (By chemical test): Solution should slightly alkaline.
Identification (By Loss on drying): NMT 1.0%
Identification (Sodium): A dense precipitate should form.
Identification (Phosphate 1): With silver nitrate solution neutral solution should yield yellow precipitate that soluble in 2N nitric acid and 6 N ammonium hydroxide.
Identification (Phosphate 2): With ammonium molybdate solution, acidified solutions should yield a yellow precipitate that should soluble in 6N ammonium hydroxide.
Identification (Phosphate 3): A yellow colour should develop
Assay (on dry basis): 98.0% - 100.5%
Assay (Dried substance): 98.0% - 101.0%
Assay (acidimetric, on dried basis): 99.0 - 101.0%
Appearance of solution: Solution should be clear and colourless.
Insoluble Substances: NMT 0.20%
Sodium dihydrogen phosphate: NMT 2.5%
pH- value (1%, water): 9.0 - 9.4
Carbonate: The solution should not effervesce.
Chlorides: NMT 0.01%
Fluoride(F): NMT 0.001%
Sulphate (SO4): NMT 0.03%
Heavy metals (as Pb): NMT 0.001%
Arsenic (As): NMT 0.0001%
Cadmium(Cd): NMT 0.0001%
Iron(Fe): NMT 0.002%
Mercury(Hg): NMT 0.0001%
Nickel (Ni): NMT 0.0002%
Lead (pb): NMT 0.0001%
Reducing substances(kmno4): The colour of the permanganate should not be completely discharge.
Loss on drying at 130°C: NMT 1.0%
Particle size (> 0.315 mm): about 99.0%
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