Glycerin USP/Ph.Eur 2LT Go Back


: 56-81-5

Molecular Formula


Molecular Weight

: 92.09
Appearance/Description: Clear, colorless, syrupy liquid, unctuous to the touch, Its solutions are neutral to litmus
Solubility: Miscible with water, ethanol(96%) and with alcohol .slightly soluble in acetone, Insoluble in fatty oils, in essential oils in chloroform, in ether, and in fixed and volatile oils.
Identification (by IR): IR spectra of the sample should be concomitant with IR spectra of the corresponding standard
Identification (Diethylene glycol): NMT 0.10%
Identification (Ethylene glycol): NMT 0.10%
Identification (By GC Chromatogram): The retention time of the glycerin peak of the sample solution corresponds to that obtained in the standard solution
Identification (By Refractive index, 20±0.5°C): 1.470 - 1.475
Identification (By Relative density): 1.258-1.268
Appearance of solution: Clear and colorless solution
Color: Color of the sample should not be darker than the color of the standard
Acidity or Alkalinity: Not more than 0.2ml of 0.1M sodium hydroxide is required to change the color of the indicator to pink.
Refractive index,20° C: 1.470 - 1.475
Specific gravity, 25°C: NLT 1.249
Aldehydes: NMT 10ppm
Impurity A (Diethylene glycol): NMT 0.1%
Related substances (Any impurity with a retention time less than the retention time of glycerol ): NMT 0.1%
Related substances: Total of all impurities with retention times greater than the retention time of glycerol: NMT 0.5%
Related compounds : Individual impurity: NMT 0.1%
Related compounds : Total impurities: NMT 1.0%
Halogenated compounds: NMT 35ppm
Sugars: The solution remains blue and no precipitate should form
Chlorides: NMT 10ppm
Sulphate (SO4): NMT 20ppm
Water: NMT 2.0%
Sulphated ash / Residue on ignition: NMT 0.01%
Assay (anhydrous substance): 99.0% - 101.0%
Chlorinated compounds: NMT 30ppm of Cl
Fatty acids and esters: NMT 1.0ml of 0.5N sodium hydroxide shall consume.
Ester: Not less than 8.0 ml of 0.1M hydrochloric acid is required to the change the color of the indicator.
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